Enamel Matrix Derivative has No Effect on the Chondrogenic Differentiation of Mesenchymal Stem Cells

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Enamel Matrix Derivative has No Effect on the Chondrogenic Differentiation of Mesenchymal Stem Cells

BACKGROUND Treatment of large bone defects due to trauma, tumor resection, or congenital abnormalities is challenging. Bone tissue engineering using mesenchymal stem cells (MSCs) represents a promising treatment option. However, the quantity and quality of engineered bone tissue are not sufficient to fill large bone defects. The aim of this study was to determine if the addition of enamel matri...

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Effects of BIO on proliferation and chondrogenic differentiation of mouse marrow-derived mesenchymal stem cells

In vitro expansion of mesenchymal stem cell (MSCs) into large number is necessary for their application in cell-based treatment of articular cartilage defects. On the other hand, some studies have indicated that BIO (6-Bromoindirubin-3-Oxime) possesses mitogenic effects on cell culture. The objective of the present study was to examine the effect of BIO on in vitro expansion and chondrogenic di...

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The effect of Dimethyl Sulfoxide on hepatogenic differentiation of Mesenchymal Stem Cells

Background Adipose tissue mesenchymal stem cells (AT-MSCs) are suitable choices in treatment of liver associated diseases. Dimethyl Sulfoxide (DMSO) is an amphipathic molecule with potential of delivering both lipophilic and hydrophilic agents into cells. Few protocols used DMSO for induction of AT-MSCs towards hepatocyte like cells but the effect of DMSO on hepatogenic differentiation were no...

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The Effect of Platelet Rich Plasma on Chondrogenic Differentiation of Human Adipose Derived Stem Cells in Transwell Culture

  Objective(s): Platelet-rich plasma (PRP) has recently emerged as a promising strategy in regenerative medicine due to its multiple endogenous growth factors. Little is known about the role of PRP as a promoter in chondrogenesis of human adipose derived stem cells (hADSCs). The aim of this study was to determine whether PRP may be considered as a natural and easy achievable source o...

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IL-17 Inhibits Chondrogenic Differentiation of Human Mesenchymal Stem Cells

OBJECTIVE Mesenchymal stem cells (MSCs) can differentiate into cells of mesenchymal lineages, such as osteoblasts and chondrocytes. Here we investigated the effects of IL-17, a key cytokine in chronic inflammation, on chondrogenic differentiation of human MSCs. METHODS Human bone marrow MSCs were pellet cultured in chondrogenic induction medium containing TGF-β3. Chondrogenic differentiation ...

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ژورنال

عنوان ژورنال: Frontiers in Bioengineering and Biotechnology

سال: 2014

ISSN: 2296-4185

DOI: 10.3389/fbioe.2014.00029